How does an injection of a filamentous phage DNA into host bacterium occur?
Attachment: Proteins in the “tail” of the phage bind to a specific receptor (in this case, a sugar transporter) on the surface of the bacterial cell. Entry: The phage injects its double-stranded DNA genome into the cytoplasm of the bacterium.
How do phages enter their host cell?
To infect bacteria, most bacteriophages employ a ‘tail’ that stabs and pierces the bacterium’s membrane to allow the virus’s genetic material to pass through.
How do filamentous phages work?
Filamentous bacteriophages contain a circular single-stranded deoxyribonucleic acid (ssDNA) genome packaged into long filaments. These phages do not reproduce by lysing bacteria; instead, they are secreted into the environment without killing the host.
How does a phage transfer DNA?
Bacteriophages mediate horizontal gene transfer through a mechanism known as transduction. Phage transduction carried out in the laboratory involves a bacterial donor and a recipient, both of which are susceptible to infection by the phage of interest.
How does an injection of a filamentous phage example M13 DNA into the host bacterium occur a via Pilus B Surface Attachment C transformation D transfection?
7. How does an injection of a filamentous phage (example- M13) DNA into the host bacterium occur? Explanation: Injection of an M13 DNA molecule into an E. coli occurs via pilus, the structure that connects two cells during sexual conjugation.
What part of the bacteriophage gets injected into a bacterial cell?
Which part of the bacteriophage was injected into the bacterial cell? The bacteriophage injects its double-stranded Deoxyribonucleic Acid (DNA) genome into the cytoplasm of the bacterial cell. Notably, the tail contains a hollow core through which the injection of DNA takes place into the host cell.
How does phage connect to bacteria?
A phage attaches to a bacterium and injects its DNA into the bacterial cell. The bacterium then turns into a phage factory, producing as many as 100 new phages before it bursts, releasing the phages to attack more bacteria.
How do phages bind to bacteria?
Generally, the infection process begins with the phage attaching to the surface of the host cell via particular host cell surface receptors. As a consequence of infection, the genetic material of the phage is injected into the cytoplasm of the bacterial cell.
When a virus attaches to a host What does it inject into the host?
During attachment and penetration, the virus attaches itself to a host cell and injects its genetic material into it. During uncoating, replication, and assembly, the viral DNA or RNA incorporates itself into the host cell’s genetic material and induces it to replicate the viral genome.
Are filamentous phages lytic?
In contrast to lytic phages, filamentous phages are constantly being extruded through the bacterial membranes without lysis. … In contrast to lytic bacteriophages, filamentous phages are assembled in the host’s membrane and extruded across the cellular envelope while the bacterium continues to grow.
What does a temperate phage do?
Temperate phages are considered as natural vectors for gene transmission among bacteria due to the ability to integrate their genomes into a host chromosome, therefore, affect the fitness and phenotype of host bacteria.
Which of the following is a filamentous phage?
The phages are named for their filamentous shape, a worm-like chain (long, thin and flexible, reminiscent of a length of cooked spaghetti), about 6 nm in diameter and about 1000-2000 nm long.
Can one bacterium insert its DNA into another bacterium?
Conjugation is a process by which one bacterium transfers genetic material to another bacterium through direct contact. During conjugation, one of the bacterial cells serves as the donor of the genetic material, and the other serves as the recipient.
Can bacteriophage lambda transfer host DNA from one bacterium to another?
Generalized and specialized transduction
It is the process by which phages can package any bacterial DNA (chromosomal or plasmid) and transfer it to another bacterium. The transducing particles of this mode of transduction form when bacterial host DNA is packaged into phage heads instead of viral DNA.
What is the name for the transfer of genetic information from one bacterium to another bacterium by a phage?
Transduction is the process by which a virus transfers genetic material from one bacterium to another. Viruses called bacteriophages are able to infect bacterial cells and use them as hosts to make more viruses.